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D5659-14 – Standard Test Method Technical Guide
ASTM D5659-14 provides a standardized test method for the determination of chlorophenoxy acid herbicides in various waste matrices using high performance liquid chromatography with ultraviolet detection (HPLC/UV). This method specifically addresses the analysis of 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), and 2,4,5-trichlorophenoxypropionic acid (Silvex).
💡 Technical Note: While the standard method is validated for a concentration range of 50 to 1000 ppm, lower detection levels can be achieved by using larger sample sizes, smaller total final volumes, or incorporating in-line or solid-phase extraction (SPE) for concentration and cleanup.